As noted in Dr. Bennett's answer, electrical charges are understood to be produced by electrons. Electrons, which are too small to be seen by eyes, are detectable individually by highly sensitive equipment. An electron is described as having, or in a sense, being, a negative electrical charge.
Early research was done calling the charge of an electron positive, but when they were able with vacuum tubes to show which direction electrical current was really going it turned out they had guessed the wrong direction, so since the side of a battery that the electric current came from was what they called "negative", it was decided to call the electron's charge "negative". Later the discovery of oppositely-charged particles in the nuclei of atoms showed how matter's charge is mostly a neutral balance. Protons don't tend to leave their atoms' nucleus much. When an atom has matching numbers of electrons and protons their charge is basically neutral. Electrons, however, leave their atoms pretty easily. Chemical reactions happen essentially with the movement of electrons, and batteries are packages of chemicals which produce electric charge by chemical means. Magnetism pushes electrons around. Electric generators use moving magnetic fields to push electrons along conductive circuits. Electrons' electric fields push against each other, and this produces an electron pressure. This pressure is called Electro-motive force (EMF), and is measured as voltage. Lots of electrons gathered together on one side of a thin insulator can push the electrons out of a conductor on the other side, causing a kind of "positive" charge on the other side.
Atoms which have lost an electron or so have a positive charge and are called positive ions. Atoms with extra electrons have a negative charge and are called negative ions. The positive charges don't make them travel through solid material but it does tend to draw electrons to them. Negative ions can deflect electrons away. All these properties are used in electronic technology.
Lightning, and static electricity built up in hair and clothing, are cases of natural electric charge buildup. They can be voltages of surprising strength. And all from bits of matter so small...