ISAC Congress-Purdue University Cytometry Mail List
rules state advertising is a Violating what happens to people that breaks the rules?
FlowJo Announcements
From: Adam Treister (adam@treestar.com)
Date: Mon Apr 22 2002 - 00:15:07 EST
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________________________________________
Only two more weeks until ISAC, that biennial bacchanalia of flower power
and fun! So I hope you'll excuse a bunch of blatantly commercial
announcements to the list, but endulge me this one time and read on.
At the show we'll be releasing FlowJo Version 4. We've got new platforms
for overlaying and clustering, we've made it work better across the
Internet, added all sorts of new conveniences, and spiffed it up with the
new OS X look and feel. What was already the best analysis software in flow
cytometry has gotten a whole lot better.
At the meeting, Tree Star is proud to be sponsoring the CyberCafe, your link
to home and responsibility while you're in carefree San Diego. Check your
email, surf the web, download the slides you'll be presenting at ten. A
cadre of California companies has contributed to bring in a premier local
roaster to satisfy all your latte urges. We hope you’ll all drop by and see
what we mean when we say we're committed to Java. The network is going
wireless this year. Just pop a 802.11 card in your laptop, and while your
neighbor plays solitaire through the keynote, you can be reading e-mail.
We're going to open the CyberCafe with the Second Biennial FlowJo Users
Group Meeting,. Saturday night May 4 at 8PM. The first user group meeting
was cancelled for lack of interest when Dave Novo brought in a case and a
half of French wine, so this year we're going to try real hard to assemble
to the point where we can see the show of hands on something before we
disband in search of alcohol. We'll have a whole bunch of Macs running
FlowJo v4 under OS X, and you can bring your own data and get into big
arguments about compensation. Be the first to get the newest FlowJo
t-shirt.
All you Windows fans out there, come by our booth to see FlowJo running on a
PC! We’ll be previewing the long awaited Java version of FlowJo. We’re
still not ready to release it, but we’ll be giving a peak as to what it is
going to do.
For those who haven't found it yet, we've unveiled a spanking new FlowJo
website. Flowjo.com is chuck full of new content, functionality and spunk.
Automated price quotes, online ordering, a FAQ that will guide you to new
depths of understanding, and none of that awful yellow on black text. The
search engine even works. No ads & cookie-free. Check it out.
Specifically, you should check our pricing. Prices are going up on May 10.
It has been a number of years since we've changed our prices and with the
development of the OSX and PC versions, it¹s time for a leap. I guess
there's no such thing as a free launch. Anyway, this may be a great time to
buy that FlowJo ten pack you've been thinking about. Licenses purchased
before May 10 are entitled to a year of free upgrades, including the 4.0
release.
Unleash the flower power!
Adam
------------------------------------------------------------------
Adam Treister
Tree Star, Inc.
ph: 800-366-6045 intl: 1-650-591-2854 fax: 1-650-508-9186
adam@treestar.com www.treestar.com
------------------------------------------------------------------
________________________________________
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• Reply: Mario Roederer: "Job Opening -- Immediate -- Vaccine Research Center"
• Reply: Michael Dustin : "MoFlo vs Vantage"
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:41:39 EST
RE: Report from ISAC Meeting San Diego
From: Adam Treister (adam@treestar.com)
Date: Wed May 22 2002 - 01:45:52 EST
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• Previous message: Simon Watson: "Computer Networks"
• Maybe in reply to: J.Paul Robinson: "Report from ISAC Meeting San Diego"
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________________________________________
> From: J.Paul Robinson
[mailto:jpr@flowcyt.cyto.purdue.edu]
> Subject: Report from ISAC Meeting San Diego
> Colleagues:
> Hello from sunny California and the ISAC XXI congress. This message comes
to you
> from the CYBER CAFE generously provided by Adam Triester of Tree Star, Inc
of FLOJO land!
> Adam has made a bank of 12 computers, a wireless network and, lots of
network cables for
> laptops. He has a T1 fast line and is providing FREE access for the
entire congress.
> The room is ALWAYS full and is definitley the most popular place in the
congress.
> It has nothing to do with the outstanding FREE coffee from Ryan Bros,
Coffee again
> generously provided by Adam. This is the best facility
provided by any vendor ever!.....
> so long live FLOJO.....and more free coffee and internet access....
Paul,
Thanks for the kind words, but I can't take all the credit for the
CyberCafe.
Apple generously provided all the Macs. IT departments around the world may
say that Macs are hard to network, but we put a dozen Macs on the Internet
in under an hour. Apple sent top of the line G4s with Cinema displays, and
Titanium PowerBooks, as well as a bunch of iMacs. Imagine what the lines
would have been like if we only had three computers, as we did in
Montpelier.
The true highlight of the cafe was the free espresso. Thanks to Phoenix Flow
Systems, Guava Technologies, PROzyme and Becton Dickinson for contributing
to the coffee fund. By the time we got to the coffee I had blown the
marketing budget on this endeavor, and these companies stepped in to make
sure you had Ryan Brothers coffee instead of the swill we'd have gotten from
the hotel. I also extend a special thanks to Kevin Becker for bringing the
Mar Dels to the banquet. The best band of any ISAC I've attended.
The CyberCafe crew was Jennifer Wilshire, Maciej Simm, Adam Treat and Amy
Hsu. They thought they were getting a leisurely week on the beach, and ended
up working 9 to 9 every day to keep the CyberCafe running. It was an
exhausting schedule, and they were tireless in their support of the
attendees' Internet needs. It never would have come off without them.
Sophia Ascani and Alexandra Treister tie-dyed the 350 shirts. Each one is a
unique work of art, and each was hand-dipped. Our garage floor has the
stains to prove it. So wear them proud, and wash them in cold water.
We've agreed to do it again at ISAC XXII in Montpelier.
I'm just thankful
this congress is only held every second year.
All this marketing crap
Just gets in the way of my programming.
Au revoir,
Adam
------------------------------------------------------------------
Adam Treister
Tree Star, Inc.
ph: 800-366-6045 intl: 1-650-591-2854 fax: 1-650-508-9186
adam@treestar.com www.flowjo.com
------------------------------------------------------------------
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:41:43 EST
RE: Descriptive statistics
From: Adam Treister (adam@treestar.com)
Date: Thu May 30 2002 - 13:11:14 EST
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• In reply to: Jan Bayer: "Re: Descriptive statistics"
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________________________________________
> Jan Bayer wrote:
> I think you should consider reading the probability binning papers,
> published sep 2001 (vol 45) in cytometry.
>
>
The important extension is that the probability binning methods include
multivariate
comparisons.
Your points are valid improvements over the well known limitations of the KS
algorithm, but they still look at only one dimension of the data. This was
exactly how we started when we set out to add comparison statistics to
FlowJo. But when we realized we should look at all of the data, and
implemented the multivariate comparisons, things worked much better.
Suddenly the software started quantifying the differences in ways that more
closely matched human assessments, and it also became able to gate on the
events that constitute the important differences.
http://www.flowjo.com/v4/html/comparison.html
Adam
------------------------------------------------------------------
Adam Treister
Tree Star, Inc.
ph: 800-366-6045 intl: 1-650-591-2854 fax: 1-650-508-9186
adam@treestar.com www.flowjo.com
------------------------------------------------------------------
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This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:41:45 EST
Re: newbie help with software compensation
From: David Novo (dave@denovosoftware.com)
Date: Fri Jun 14 2002 - 00:24:27 EST
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• In reply to: Marty Bigos: "Re: newbie help with software compensation"
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________________________________________
Hi Marty,
Just to reassure you, FCS Express does use the linear model correctly. It may shock you
to learn that other people, aside from your friends at Treestar, can read Dr. Bagwell's
paper and actually understand it enough to implement the algorithms that he describes.
FCS Express allows you to enter the files with the single stained controls and it
calculates the matrix values based upon the data in the file. In addition, in order to
see how slight changes in the compensation can distort your data, you can change the
compensation values with sliders to see what happens. Its a great for teaching people
the difference between proper, over- and under- compensated data. You can also enter
a user defined value to smooth out the digitization errors that can occur in the low
end of lower resolution data.
If anyone finds any problems with the compensation (which hasn't happened yet), please
let me know and of course I will fix any errors. But until that happens, I think that
your assumption that FCS Express uses the linear model correctly is actually a very
good one.
-Dave
At 06:23 PM 6/12/02 -0700, you wrote:
>Lastly, of course, this assumes that Summit (and FCS-Express) do use
>the linear model correctly. In an older version of Summit I used,
>overcompensation resulted in data "bouncing" off the axis instead of
>being smashed down on the axis.
________________________________________
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:41:48 EST
Advertising
From: Mario Roederer (roederer@drmr.com)
Date: Fri Nov 01 2002 - 16:46:47 EST
• Next message: William Telford: "Re: Fluorochromes choice"
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• Next in thread: Penney Robbins: "RE: Advertising"
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________________________________________
I agree that there have been far too many commercial-oriented emails
on the list. I appreciate the efforts of most manufacturers to
withhold from advertising on this list (plus, of course, the efforts
of Steve & Paul to filter those out).
The list, which is an outstanding forum for exchange of information,
has been occasionally used to identify substantially new products
which can significantly impact on how we do experiments. I feel that
the Molecular Probes email of 10/30 clearly does not fall into this
category; the new product advertised was no more than a slight
modification of the existing one. Such an email should be directed
solely to the person requesting information; if that person then
collates responses and puts it back on the list then so be it. But
for manufacturers to directly respond in this way is
counter-productive to the goals of this list.
I would like to propose a 6-month moratorium on all emails that are
no more than advertisements. Note that I write "would like
to"--because I'm not sure that this is possible. I don't want to put
any additional onus on Steve or Paul to filter out the borderline
emails. While these may be easy to identify when they come from
manufacturers, it could just as well be considered blatant
advertising when they come from a user.
Therefore, perhaps we can see if the commercial participants on this
list could exercise self-restraint rather than requesting a formal
censorship of advertising emails.
Thus, if you are a manufacturer, and you are responding to somebody's
request for information, do so privately to that person ONLY. It is
up to the person requesting information to decide whether or not the
information received in response to the query warrants a summary on
the list.
If you are not a manufacturer, and are responding to somebody's
request for specific information, please consider whether your
response (that identifies a specific product or manufacturer) is of
general enough interest to warrant the list. If it does not, then
simply send it privately to the person who requested information, and
let them decide whether to post the summary of responses.
In general, I urge people to err on the side of caution and send
their information only to the person who requested it. Realize that
if several people want the same information, they can always request
it from the original poster! I have posted queries to the list;
people have sent me emails asking me to forward to them the
responses, which I did.
This process can significantly cut down on emails that might be
viewed as too commercial.
mr
________________________________________
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:42:08 EST
advertizing - no need to comment on this please.
From: J.Paul Robinson (jpr@flowcyt.cyto.purdue.edu)
Date: Sat Nov 02 2002 - 17:43:44 EST
• Next message: Mike Wade: "Rat p53 antibody"
• Previous message: cy: "CD34"
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________________________________________
>From the list-owner:
Please don't start another "he said, she said" on advertizing. I don't want to
have to spend half my day negotiating squabbles between intelligent people
(and scientists)
Lets keep it down to a dull roar. This is a discussion group whose intentions
are to facilitate information exchange and knowledge .
OK that's enough. I hope there will not be a host of comments on this. Get
back to the science.....
thanks
Paul Robinson
Purdue University
J.Paul Robinson, PhD PH:(765)4940757
Professor of Immunopharmacology
Professor of Biomedical Engineering
Purdue University FAX:(765)4940517
EMAIL:jpr@flowcyt.cyto.purdue.edu
WEB: http://www.cyto.purdue.edu
________________________________________
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This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:42:08 EST
RE: Advertising
From: Penney Robbins (parneogen@excite.com)
Date: Mon Nov 04 2002 - 15:44:10 EST
• Next message: Richard Haugland: "Re: Fluorochromes choice"
• Previous message: Beavis, Andrew: "Positions Available at Esoterix Inc, New Jersey"
• Maybe in reply to: Mario Roederer: "Advertising"
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________________________________________
Well, I disagree. There aren't that many commerical e-mails, and for us, an isolated
startup company, separated from academic institutions, one of the commerical e-mails
was helpful, we would not have know about the product otherwise (see I am even not
going to advertise it!).
All best,
Penney Robbins, PhD.
--- On Fri 11/01, Mario Roederer wrote:
From: Mario Roederer [mailto: roederer@drmr.com]
To: cyto-inbox
Date: Fri, 1 Nov 2002 16:46:47 -0500
Subject: Advertising
>
> I agree that there have been far too many commercial-oriented emails
> on the list. I appreciate the efforts of most manufacturers to
> withhold from advertising on this list (plus, of course, the efforts
> of Steve & Paul to filter those out).
>
> The list, which is an outstanding forum for exchange of information,
> has been occasionally used to identify substantially new products
> which can significantly impact on how we do experiments. I feel that
> the Molecular Probes email of 10/30 clearly does not fall into this
> category; the new product advertised was no more than a slight
> modification of the existing one. Such an email should be directed
> solely to the person requesting information; if that person then
> collates responses and puts it back on the list then so be it. But
> for manufacturers to directly respond in this way is
> counter-productive to the goals of this list.
>
> I would like to propose a 6-month moratorium on all emails that are
> no more than advertisements. Note that I write "would like
> to"--because I'm not sure that this is possible. I don't want to
> put
> any additional onus on Steve or Paul to filter out the borderline
> emails. While these may be easy to identify when they come from
> manufacturers, it could just as well be considered blatant
> advertising when they come from a user.
>
> Therefore, perhaps we can see if the commercial participants on this
> list could exercise self-restraint rather than requesting a formal
> censorship of advertising emails.
>
> Thus, if you are a manufacturer, and you are responding to somebody's
> request for information, do so privately to that person ONLY. It is
> up to the person requesting information to decide whether or not the
> information received in response to the query warrants a summary on
> the list.
>
> If you are not a manufacturer, and are responding to somebody's
> request for specific information, please consider whether your
> response (that identifies a specific product or manufacturer) is of
> general enough interest to warrant the list. If it does not, then
> simply send it privately to the person who requested information, and
> let them decide whether to post the summary of responses.
>
> In general, I urge people to err on the side of caution and send
> their information only to the person who requested it. Realize that
> if several people want the same information, they can always request
> it from the original poster! I have posted queries to the list;
> people have sent me emails asking me to forward to them the
> responses, which I did.
>
> This process can significantly cut down on emails that might be
> viewed as too commercial.
>
> mr
>
_______________________________________________
Join Excite! - http://www.excite.com
The most personalized portal on the Web!
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:42:08 EST
Re: advertising
From: Alice L. Givan (Alice.L.Givan@dartmouth.edu)
Date: Tue Nov 05 2002 - 17:52:37 EST
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________________________________________
I would not like for Dick Haugland to feel that we are criticizing his participation in
this list. Molecular Probes has been a HUGE help to all flow and imaging cytometrists.
Advice from that company has been good, generous, dependable, and without strings.
The new MP handbook is a fantastic source of information. I would hope that advice
from Dick will continue to be submitted to the whole network --- and if he gets a bit
of free advertising from it, then he deserves it.
Alice
Alice L. Givan
Englert Cell Analysis Laboratory
of the Norris Cotton Cancer Center
Dartmouth Medical School
Lebanon, New Hampshire NH 03756
tel 603-650-7661
fax 603-650-6130
givan@dartmouth.edu
________________________________________
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:42:08 EST
Re: advertising
From: Alice L. Givan (Alice.L.Givan@dartmouth.edu)
Date: Wed Nov 06 2002 - 14:38:54 EST
• Next message: Amy Raber: "degranulation-another question!"
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________________________________________
I need to add that I was NOT suggesting that this network give any special dispensation
to Dick Haugland and Molecular Probes. They, like many other companies, are of
great benefit to the flow community. I was suggesting that we be a bit relaxed in
our willingness to accept subtle advertising in general --- because we can benefit
from being able to get advice directly from people who are at the forefront of flow
development. Obviously, knowing where to draw the line can be difficult.....
Alice
Alice L. Givan
Englert Cell Analysis Laboratory
of the Norris Cotton Cancer Center
Dartmouth Medical School
Lebanon, New Hampshire NH 03756
tel 603-650-7661
fax 603-650-6130
givan@dartmouth.edu
________________________________________
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:42:08 EST
Re: advertising
From: Robb Habbersett (robb@lanl.gov)
Date: Wed Nov 06 2002 - 14:19:13 EST
• Next message: Richard Haugland: "Re: rat blood eosinophils"
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• Reply: Marty Bigos: "Re: advertising"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]
________________________________________
Once again Alice is ... RIGHT ON!!
Robb
At 05:52 PM 11/5/2002 -0500, Alice L. Givan wrote:
>I would not like for Dick Haugland to feel that we are criticizing his
>participation in
>this list. Molecular Probes has been a HUGE help to all flow and imaging
>cytometrists.
>Advice from that company has been good, generous, dependable, and without
>strings.
>The new MP handbook is a fantastic source of information. I would hope
>that advice
>from Dick will continue to be submitted to the whole network --- and if he
>gets a bit
>of free advertising from it, then he deserves it.
>
>Alice
>
>Alice L. Givan
>Englert Cell Analysis Laboratory
>of the Norris Cotton Cancer Center
>Dartmouth Medical School
>Lebanon, New Hampshire NH 03756
>tel 603-650-7661
>fax 603-650-6130
>givan@dartmouth.edu
Robert C. Habbersett
Technical Staff Member
Los Alamos National Laboratory
MS-M888 BN2
Los Alamos, NM 87545
phone 505.667.0296
fax 505.665.3024
"As often happens in science, the paradox was resolved as soon as the
obvious was abandoned in the face of experimental evidence."
{from a recent review in Science}
________________________________________
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• Reply: Marty Bigos: "Re: advertising"
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:42:09 EST
Re: advertising
From: Marty Bigos (mbigos@gladstone.ucsf.edu)
Date: Thu Nov 07 2002 - 18:44:09 EST
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________________________________________
I second (or probably 259th by the time my slow mail server gets this
out) this sentiment. Marty
>Once again Alice is ... RIGHT ON!!
>
>Robb
>
>At 05:52 PM 11/5/2002 -0500, Alice L. Givan wrote:
>
>>I would not like for Dick Haugland to feel that we are criticizing his
>>participation in
>>this list. Molecular Probes has been a HUGE help to all flow and imaging
>>cytometrists.
>>Advice from that company has been good, generous, dependable, and without
>>strings.
>>The new MP handbook is a fantastic source of information. I would hope
>>that advice
>>from Dick will continue to be submitted to the whole network --- and if he
>>gets a bit
>>of free advertising from it, then he deserves it.
>>
>>Alice
>>
>>Alice L. Givan
>>Englert Cell Analysis Laboratory
>>of the Norris Cotton Cancer Center
>>Dartmouth Medical School
>>Lebanon, New Hampshire NH 03756
>>tel 603-650-7661
>>fax 603-650-6130
>>givan@dartmouth.edu
>
>Robert C. Habbersett
>Technical Staff Member
>Los Alamos National Laboratory
>MS-M888 BN2
>Los Alamos, NM 87545
>phone 505.667.0296
>fax 505.665.3024
>"As often happens in science, the paradox was resolved as soon as the
>obvious was abandoned in the face of experimental evidence."
>{from a recent review in Science}
________________________________________
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:42:09 EST
EMAIL ABUSE - how to stop
From: J.Paul Robinson (jpr@flowcyt.cyto.purdue.edu)
Date: Thu Dec 12 2002 - 08:43:08 EST
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________________________________________
Colleagues: I am sending out a copy of a message I have just sent to
RNWAY laboratories of South Korea and all 20 worldwide distributors of
RNWAY products most of whom are highly reputable companies. I am only
sending it to you because I am going to propose to create a small
"SCIENTISTS against EMAIL ABUSE" type of revolutionary action.........
I won't send you more copies of what I am going to send out, but I will post
them in a prominent place on our website so that you can follow and
participate in the action.
I am truly sick and tired of receiving literally dozens of unsolicited crap
messages each day. However, receiving them from supposedly reputable
companies drives me really crazy. These, I can do something about and I am.
The rest are virtually beyoned any reasonable solution. As you can see, I have
started with RNWAY, a company that I have requested a dozen times to
remove me from their abusive lists. This week they sent me an attachment to
boot....- something they do constantly. This made me really mad.
Perhaps it was the beef I ate in the UK this week, but I got really mad when
RNWAY filled my box yet again.....and I am going to set up a place where
people can register copies of messages they send recording their requests
for removal. This record will become a documentation place you can copy
your messages to for any future legal actions. We will code the email
address of the requestor so you don't get further abuse, but we will publish
the list of companies that continue to abuse us.
If you have suggestions, please let me know.
Sincerely
"Mad Cow" Paul Robinson
Purdue University
---------------------------------------------------------------------------
Here is the email that I sent to RNWAYs 20 worldwide distributors
---------------------------------------------------------------------------------------------
You are receiving this message if you are associated with RNWAY products.
We have continually requested removal from RNWAYS abusive and constant
emails. It is against Federal law in the United States to continue to send
unsolicited emails after requests to discontinue are made. RNWAY posts a
privacy policy that they make no attempt to enforce. Our requests have been
documented by the university on numerous occasions. RNWAY is now liable
in the United States for severe penalties under US law. As a distributor of this
company you may also incur loss of trade, or reputation for the actions we are
going to take.
As scientists who buy your products, we are going to exert our own economic
power. We will be letting members of our various associated networks that
RNWAY will be listed as an EMAIL SPAM ABUSER. We will be
recommending that all of our users do not purchase any of their products if
they continue to abuse our email accounts.
As a scientist who uses the INTERNET as a crucial component of my
communication with others, I am sick of abuse of my email and I have
decided that I am going to do something about it, one company at a time.
This message has been copied to the 20 companies that distribute RNWAY
products around the world. This is the first of a series of actions. It will also be
copied to 3000 scientists who may have an interest in stopping this type of
abuse. It will also be posted ona public site that had last year over 5 million
access. If you as a distributor want to make a profit on selling (scientists)
products, I suggest you ensure that the comanies you deal with don't abuse
us. If they do, we won't buy their products and you will lose money. Do you
hear us?
Yours sincerely
J.Paul Robinson, Professor, Purdue University
------------------------------------------------
J.Paul Robinson, PhD PH:(765)4940757
Professor of Immunopharmacology
Professor of Biomedical Engineering
Purdue University FAX:(765)4940517
EMAIL:jpr@flowcyt.cyto.purdue.edu
WEB: http://www.cyto.purdue.edu
________________________________________
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This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:42:13 EST
Re: EMAIL ABUSE - how to stop
From: Adam Treister (adam@treestar.com)
Date: Mon Dec 16 2002 - 16:00:07 EST
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________________________________________
On Thursday, December 12, 2002, at 05:43 AM, J.Paul Robinson wrote:
>
> Colleagues: I am sending out a copy of a message I have just sent to
> RNWAY laboratories of South Korea and all 20 worldwide distributors of
> RNWAY products most of whom are highly reputable companies. I am only
> sending it to you because I am going to propose to create a small
> "SCIENTISTS against EMAIL ABUSE" type of revolutionary action.........
>
Paul,
Sounds like you're advocating fighting disease by eradicating the
antigen instead of boosting the immune response. You can organize all
you want on eliminating the pest, but until they put a stamp tax on
email, a better approach is to let the
messages be out there, but have them filtered to oblivion before you
ever see them.
In your case, the postmaster at Purdue is probably already filtering
millions of messages a day that come to the thousands of email users on
campus. They are probably capable of shutting down any RNWAY mail, and
spreading the word to other postmasters that they also should filter
those messages.
So if you can get the IT people at the university to tighten their
sieve, that's best. Otherwise you have to switch to an email program
that has good junk filters. I think I get 500+ messages a day, and
only 10 to 20 make it past the junk filter.
Until last summer I was using Outlook Express and spam was a huge
problem. Since then I switched to the free Mail program in OS X, which
just added special features for spam detection and removal. Its
probably 97% effective, and I haven't found any false positives.
So, of course, the best answer is to get a Mac :)
I'm sure the PC mail clients are addressing this issue as well. I
believe there are central databases of offenders so programs can learn
from others which messages to delete. I would imagine this is the
most important feature in any email program sold these days, so I bet
Eudora or other third party mail programs have this solved.
There's a lot of information on the subject at:
http://spam.abuse.net/
Whether you fight the problem on the server or the client, it
definitely is worth getting it cleaned up. I found it screwed up my
whole communications process because every time I wanted check email, I
had to wade through dozens or hundreds of useless ads.
Adam
---------------------------------------------------
Adam Treister
adam@treestar.com
www.flowjo.com 800-366-6045
---------------------------------------------------
________________________________________
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Re: DNA analysis softwares
From: Adam Treister (adam@treestar.com)
Date: Thu Jun 10 1999 - 22:43:01 EST
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________________________________________
----->>> On 10-Jun-1999, Ng Bee Ling wrote:
> I am looking for a software so as to determine the % of cell cycle
> phases (with gating properties) from listmode files. Is there such
> software that could run both for files collectd from EXPO and
> Cellquest?
--------------
FlowJo in an offline analysis package that will read both EXPO (PC) and
CellQuest (Mac) files. You can compute the percentages in each of the
phases (as well as under-G1 and over-G2) for any gated population.
We currently have a limitation in the ability to export all of the cell
cycle statistics for all of the samples to a spreadsheet in a single step,
but that is implemented in the next release.
More info at:
http://www.treestar.com/flowjo/html/cellcycle.html
I get a lot of requests for the references to the models, so let me give
them here:
1) Watson Pragmatic
Cytometry 8:1-8 (1987)
Watson, Chambers, & Smith: A Pragmatic Approach to the Analysis of DNA
Histograms with a Definable G1 Peak
2) Dean Jett Fox
Cytometry 1:71-80 (1980)
Fox: A Model for the Computer Analysis of Synchronous DNA Distributions
Obtained by Flow Cytometry
3) Two Populations
Uses Dean-Jett model (with Fox modification) for both populations.
Adam
-----------------------------
Adam Treister
adam@treestar.com
http://www.treestar.com/flowjo
800-366-6045
-----------------------------
________________________________________
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This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:37:11 EST
Re: EPICS and Y2K ( summer special !)
Summer of 99 special -- FlowJo for a year for $99
From: Adam Treister (adam@treestar.com)
Date: Thu Jul 29 1999 - 15:00:16 EST
• Next message: Keith Bahjat: "Re: facs archive media"
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• Maybe in reply to: Bassem Allam: "EPICS and Y2K"
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________________________________________
----->>> On 29-Jul-1999, Bassem Allam wrote:
> Hi flowers,
> We have a young EPICS C flow cytometer (just 10 years old)
> which is in pretty good shape, an we plan to keep it active
> for the next couple of years. In these machines, the date is
> configured daily using 2 digits. That why I'm wondering if
> someone have any idea about problems that may occur related
> to year 2000. Does there any reason to worry about that ??
> Thanks in advance
--------------
I'm working on an FCS file editor, one function of which will be
an automated date changing function. So if there are Y2K problems
you can set back the date on the cytometer, and then change all
the files in one shot after collection.
This will be a free utility, and will do other keyword editing too,
but a big motivation is just to provide a quick and dirty solution
to the Y2K issues that will affect many of the cytometers out there.
The design spec is to just add a user-specified number of years
to the $DATE keyword, and to write it back to the file (using
4 digits for the year). The original date field will be put in a
different keyword, and the program will also be able to restore the
file to its original state. If anyone has more elaborate requirements
than that, I'd appreciate it if you'd drop me a note.
Adam
-----------------------------
Adam Treister
adam@treestar.com
Summer of 99 special -- FlowJo for a year for $99
http://www.treestar.com/flowjo/summer99.html
650-508-9349
-----------------------------
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:37:18 EST
Adam triester and Dave Novo advertising a $99 promo
Clarifacation of what DAVE NOVO stated on Flowjo platform
Re: Cost-benefit ratio of FlowJo [Clarification]
From: Adam Treister (adam@treestar.com)
Date: Wed Aug 04 1999 - 16:28:49 EST
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________________________________________
If I may clarify...
Judging from the phone calls I'm getting, Cariappa may
have implied that FlowJo is a cross-platform package.
I think he meant that FlowJo reads data collected on any
cytometer, whether it was hooked to HP, PC or Mac. FlowJo
itself still runs only on the Mac.
This may be unclear, because we are running a Summer
$99 promotion, in conjunction with DeNovo Software
. They sell the PC based analysis package: FCS Express. So, the
solution I can offer you for Windows is at:
<http://www.denovosoftware.com/>
The promotion is a good deal on either platform.
We are working on Win-FlowJo, but it's not close to ready.
The bad news: there is no announced release planned for
a Windows product in the foreseeable future.
The good news: more for the Mac is coming pretty soon.
Adam
----->>> On 4-Aug-1999, Cariappa Annaiah wrote:
>
> I routinely obtain flow data from both Mac and PC based
> machines and so have some experience using Mac/PC data
> analysis software. I have always found it a pain to switch
> from Mac based software to PC based software and vice versa.
> That pain is history with FlowJo! The cross-platform
> capability and the ability to do in-depth, multi-tube
> analysis has made FlowJo an important member of my pantheon
> of FCM data analysis software. Now that it is being offered
> at a really low price for a limited time (as a promotion),
> the cost-benefit ratio for FlowJo becomes very favourable,
> compared to extant FCM software. Check it out at the
> following URL: http://www.treestar.com/flowjo/summer99.html
--------------
-----------------------------
Adam Treister
adam@treestar.com
http://www.treestar.com/flowjo
800-366-6045
-----------------------------
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This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:37:18 EST
Report from ISAC Meeting San Diego
From: J.Paul Robinson (jpr@flowcyt.cyto.purdue.edu)
Date: Wed May 08 2002 - 15:24:55 EST
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________________________________________
________________________________________
•
• Colleagues:
•
• Hello from sunny California and the ISAC XXI congress. This message
• comes to you from the CYBER CAFE generously provided by Adam Triester
• of Tree Star, Inc of FLOJO land! Adam has made a bank of 12 computers, a
• wireless network and, lots of network cables for laptops. He has a T1 fast
• line and is providing FREE access for the entire congress. The room is
• ALWAYS full and is definitley the most popular place in the congress. It has
• nothing to do with the outstanding FREE coffee from Ryan Bros, Coffee
• again generously provided by Adam. This is the best facility provided by any
• vendor ever!.....so long live FLOJO.....and more free coffee and internet
• access....
•
•
• This message contains several important pieces of information including
• information about the proposed Cytomics initiative that Professor Paul Smith
• has established. For link, see the end of this message.
•
•
• This is the last year that I have served as a councilor so I want to thank you
• all for the opportunity of serving on this important committee. I highly
• recommend those who are interested in participating in the society
• management to seek election to these posts. I want to congratulate the new
• council members and express my thanks to those leaving their posts. Lisa
• Staiano-Coico has done a great job as President and we thank her for her
• dedication. Maria Pallavicini has been elected as the new president and we
• congratulate her. She will be running the next congress in Montpellier
• France. Congratulations to Frank Traganos was elected Treasure to replace
• Maria. Harry Crissman has done an outstanding job of organizing this
• meeting, which by the way, has attracted a record 1100 plus attendees.
•
•
• New councilors are Paul Smith, Jan Gratama, John Nolan and Bob Zucker.
•
•
• I want to draw your attention to an important opportunity that Paul Smith is
• organizing. It is known as the CYTOMICS EU FRAMEWORK 6 INITIATIVE. if
• you are interested in participating in ANY way - from anywhere in the world,
• please go to the Purdue main page and click on the GREEN button. Enter
• your info and we will contact you. This is NOT an obligation, only an
• expression of interest in participating. Please do this URGENTLY as we
• need the info within a couple of weeks. The direct link is
•
• <underline><color><param>0000,8000,0000</param>http://www.cyto.purdue.edu/flowcyt/eucytomics/</underline><color><param>0000,0000,0000</param>
•
•
• Best Regards
•
• Paul Robinson
•
• Purdue
•
• J.Paul Robinson, PhD PH:(765)4940757
•
• Professor of Immunopharmacology
•
• Professor of Biomedical Engineering
•
• Purdue University FAX:(765)4940517
•
• EMAIL:jpr@flowcyt.cyto.purdue.edu
•
• WEB: http://www.cyto.purdue.edu
•
• <nofill>
• J.Paul Robinson, PhD PH:(765)4940757
• Professor of Immunopharmacology
• Professor of Biomedical Engineering
• Purdue University FAX:(765)4940517
• EMAIL:jpr@flowcyt.cyto.purdue.edu
• WEB: http://www.cyto.purdue.edu
•
•
• -- End --
• text/enriched attachment: stored
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:41:41 EST
Announcing User Forum at Verity Software House, Inc.
From: VSH - Tech Support (tech@vsh.com)
Date: Tue Mar 12 2002 - 11:17:49 EST
• Next message: Adrian Smith: "UV excitable dyes for surface phenotyping - ELF-97?"
• Previous message: bernadette bellette: "[TLR2 and 4 on murine Langerhans Cells?]"
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________________________________________
Hello, Flow-ers,
Verity Software House is pleased to announce the creation of a Verity User's
Forum on our web site at www.vsh.com/forum. Explore features, discuss
applications, and share "tricks of the trade" with new and experienced users
alike! With a vast user network, answers and ideas are only a few clicks
away. Stop by and say hello, and we'll all benefit from our collective
experience. It's another example of Verity's commitment to customer
support!
Have a great day everyone!
Don
Donald J. Herbert
Technical Support Manager
Verity Software House, Inc.
PO Box 247
45A Augusta Road
Topsham, ME, USA 04086
Phone: (207) 729-6767 ext.190
Fax: (207) 729-5443
email: tech@vsh.com
web: www.vsh.com
________________________________________
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:41:30 EST
Re: Wich differences between Cell Quest and Flow Jo?
From: Adrian Smith (A.Smith@centenary.usyd.edu.AU)
Date: Mon Mar 04 2002 - 19:52:55 EST
• Next message: Van Bockstaele, Dirk: "RE: Bascteria sorting ?"
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• In reply to: Simona Ronzoni: "Wich differences between Cell Quest and Flow Jo?"
• Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ]
________________________________________
>Dear Flowers,
>I always work with Cell Quest but now I downloaded Flow Jo program
>and I have a problem.
>I don't know where is the option for create the union of two gates (
>In Cell Quest is R1 or R2)
>Then I would to know wich are the substantial differences between
>Cell Quest and Flow Jo
>Thanks in advance
>Ciao
>Simona
FlowJo uses a hierarchical rather than a logical gate structure, ie
you don't work by creating two gates and then combining them, rather
you place the first gate, open the gated plot and place the second
gate directly on that plot.
The best way to get into FlowJo is to do the tutorial that can be
downloaded from the web-site (<http://www.flowjo.com/tutorial.html>).
Until you have got your head around the fundamental differences
between the programs it is very hard to compare them.
Adrian
________________________________________
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:41:27 EST
FlowJo Analysis Seminar
From: Martha Kirby (mkirby@nhgri.nih.gov)
Date: Mon Apr 08 2002 - 15:51:42 EST
• Next message: Voorn, J.: "conclusions to the ELISPOT interest inventory"
• Previous message: Kurtz, James: "[7AAD viability for CD34]"
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________________________________________
To Flow Cytometry Group,
Hi! Just wanted to let you know that Jennifer Wilshire from Tree
Star, Inc. will be giving a FlowJo Software Demonstration this
Thursday, April 11 at 1:00 PM in the 4th Floor Conference Room (room
4A46), Bldg. 49, NIH, NHGRI. This Flow Cytometry Analysis Seminar is
open to all interested.
Thanks,
Martha
Disclaimer of Endorsement: Reference herein to any specific
commercial products, process, or service by trade name, trademark,
manufacturer, or otherwise, does not necessarily constitute or imply
its endorsement, recommendation, or favoring by the United States
Government. The views and opinions of authors expressed herein do not
necessarily state or reflect those of the United States Government,
and shall not be used for advertising or product endorsement purposes.
--
Martha Kirby
NIH, NHGRI, GMBB
Bldg. 10, Rm. 2C19
9000 Rockville Pike
Bethesda, MD 20892
301 402-2264
mkirby@NHGRI.NIH.GOV
________________________________________
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________________________________________
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:41:36 EST
